세부과제번호
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2016M3A9D5A01952416
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단계
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2단계 1차년도
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세부과제명
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유전자변형마우스 병리표현형 분석서비스시스템 구축 및 운용
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공동 유/무
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Y
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SCI여부
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Y
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게재년월
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2017-01
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논문제목
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In vivo near-infrared imaging and phototherapy of tumors using a cathepsin B-activated fluorescent probe.
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총저자명
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Xiaoqiang Chen, Dayoung Lee, Sungsook Yu, Gyoungmi Kim, Songyi Lee, Yejin Cho, Haengdueng Jeong, Ki Taek Nam, Juyoung Yoon
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학술지명
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Biomaterials
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게재권(호)
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122()
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저널구분
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-
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페이지수
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130-140
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참여연구원
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-
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연구책임자
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남기택
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과제기여도
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50
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PMID
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28113107
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사사기관수
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-
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IF (년도)
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8.402
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제1저자
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Xiaoqiang Chen
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교신저자
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Juyoung Yoon
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공동저자
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Dayoung Lee, Sungsook Yu, Gyoungmi Kim, Songyi Lee, Yejin Cho, Haengdueng Jeong, Ki Taek Nam
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초록
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The development of multifunctional reagents for simultaneous specific near-infrared (NIR) imaging and phototherapy of tumors is of great significance. This work describes the design of a cathepsin B-activated fluorescent probe (CyA-P-CyB) and its applications as an NIR imaging probe for tumor cells and as a phototherapy reagent for tumors. In vitro experiments demonstrated that CyA-P-CyB was activated via the cleavage of a peptide linker by cathepsin B in tumor cells to produce fluorescence in the NIR region based on a FRET mechanism. MTT assays showed that the phototoxicity of CyA-P-CyB toward cells depended on the activity of cathepsin B, and the probe exhibited specific phototoxicity toward tumor cells. CyA-P-CyB was also successfully applied to the in vivo imaging and phototherapy of tumors. Histological analysis indicated that CyA-P-CyB had no cytotoxic effects on seven mouse tissues (lung, liver, heart, kidney, pancreas, spleen and brain) after the CyA-P-CyB treatment and laser irradiation.
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