연구논문

세부과제번호 2014M3A9D5A01075129 단계 2단계 1차년도
세부과제명 야생유래신규 근교계 마우스 개발 공동 유/무 N
SCI여부 N 게재년월 2017-03
논문제목 Over-expression of myosin 7A in cochlear hair cells of circling mice
총저자명 Yoo Yeon Kim, Hajin Nam, Harry Jung, Boyoung Kim, and Jun Gyo Suh
학술지명 Lab Anim Res 게재권(호) 33(1)
저널구분 - 페이지수 1-7
참여연구원 - 연구책임자 서준교
과제기여도 80 PMID -
사사기관수 - IF (년도) -
제1저자 Yoo Yeon Kim 교신저자 Jun Gyo Suh
공동저자 -
초록
Circling mouse (C57BL/6J-cir/cir) deleted the transmembrane inner ear (Tmie) gene is an animal model for human non-syndromic recessive deafness, DFNB6. In circling mouse, hair cells in the cochlea have degenerated and hair bundles have become irregularity as time goes on. Tmie protein carries out a function of the mechanoelectrical transduction channel in cochlear hair cells. Myosin7a (MYO7A) protein has key roles in development of the cochlear hair bundles as well as in the function of cochlear hair cells. To find whether Tmie protein interacts with MYO7A proteins in the cochlea postnatal developmental stage, we investigated expression of the MYO7A proteins in the cochlear hair cells of circling mice by western blot analysis and whole mount immunofluorescence at postnatal day 5 (P5). The expression of MYO7A showed statistically significant increase in the cochlea of C57BL/6J-+/cir and C57BL/6J-cir/cir mice than that of C57BL/6J-+/+ mice. The MYO7A intensity of the cochlear hair cells also increased in C57BL/6J-+/cir and C57BL/6J-cir/cir mice compared with those of C57BL/6J-+/+ mice. Taken together, the results indicate that Tmie protein may have an important role with MYO7A protein in the development and maintenance of the stereociliary bundles during postnatal developmental stage of the cochlea.
다음글
이전글