연구논문

세부과제번호 2014M3A9D5A01074813 단계 1단계 3차년도
세부과제명 마우스 자원 및 표현형 분석 서비스 인프라 구축 공동 유/무 -
SCI여부 N 게재년월 2016-04
논문제목 Rapid and efficient identification of the mouse leptin receptor mutation (C57BL/KsJ-db/db) by tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) analysis.
총저자명 Harry JungHajin NamJun-Gyo Suh
학술지명 Lab Anim Res 게재권(호) 32(1)
저널구분 - 페이지수 70-73
참여연구원 윤여성 연구책임자 윤여성
과제기여도 50 PMID 27051445
사사기관수 - IF (년도) -
제1저자 정해리 교신저자 서준교
공동저자 -
초록
The C57BLKS/J-Lepr(db) mouse has a point mutation in the leptin receptor gene and is one of the most useful animal model for non-insulin dependent diabetes mellitus in human. Since the homozygote of C57BLKS/J-Lepr(db) mouse is infertile, detection of point mutation in the leptin receptor gene is important for efficient maintaining strains as well as mass production of homozygotes. To develop a rapid and efficient genotyping method for C57BLKS/J-Lepr(db) mouse, the tetra-primer amplification-refractory mutation system polymerase chain reaction (ARMS-PCR) was used. The 407 and 199 bp PCR products were amplified from normal (+/+) mice; while the 407 and 268 bp PCR products were amplified from homozygotes (db/db) mice; and the 407, 268, and 199 bp PCR products were amplified from heterozygotes (db/+) mice. This result showed that the tetra-primer ARMS-PCR analysis by us is suitable to detect point mutation of the leptin receptor gene. Taken together, our method will dramatically reduce animal use for maintenance of strains as well as production of homozygote in the C57BLKS/J-Lepr(db) strains.
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