연구논문

세부과제번호 2013-31-0676 단계 1단계 1차년도
세부과제명 감각기 표현형 분석 공동 유/무 -
SCI여부 Y 게재년월 2014-03
논문제목 Cyanine-based fluorescent probe for highly selective detection of glutathione in cell cultures and live mouse tissues
총저자명 Jun Yin, Younghee Kwon, Dabin Kim, Dayoung Lee, Gyoungmi Kim, Ying Hu, Ji-Hwan Ryu, Juyoung Yoon
학술지명 Journal of the american chemical society 게재권(호) 136(14)
저널구분 국외 페이지수 11.444
참여연구원 - 연구책임자 서경률
과제기여도 - PMID
사사기관수 IF (년도)
제1저자 교신저자
공동저자
초록
Glutathione (GSH) plays a crucial role in human pathologies. Near-infrared fluorescence-based sensors capable of detecting intracellular GSH in vivo would be useful tools to understand the mechanisms of diseases. In this work, two cyanine-based fluorescent probes, 1 and 2, containing sulfonamide groups were prepared. Evaluation of the fluorescence changes displayed by probe 1, which contains a 2,4-dinitrobenzenesulfonamide group, shows that it is cell-membrane-permeable and can selectively detect thiols such as GSH, cysteine (Cys), and homocysteine (Hcy) in living cells. The response of 1 to thiols can be reversed by treatment with N-methylmaleimide (NMM). Probe 2, which possesses a 5-(dimethylamino)naphthalenesulfonamide group, displays high selectivity for GSH over Cys and Hcy, and its response can be reversed using NMM. The potential biological utility of 2 was shown by its use in fluorescence imaging of GSH in living cells. Furthermore, probe 2 can determine changes in the intracellular levels of GSH modualated by H2O2. The properties of 2 enable its use in monitoring GSH in vivo in a mouse model. The results showed that intravenous injection of 2 into a mouse generates a dramatic image in which strong fluorescence is emitted from various tissues, including the liver, kidney, lung, and spleen. Importantly, 2 can be utilized to monitor the depletion of GSH in mouse tissue cells promoted by excessive administration of the painkiller acetaminophen. The combined results coming from this effort suggest that the new probe will serve as an efficient tool for detecting cellular GSH in animals.
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